What is HSE? 

Every person has two copies of their genome, one from their mother and father.  With the exception of gametes, DNA is present in each cell of the body in this diploid state.  Minor differences (polymorphisms) between the two copies of the genome can lead to ambiguities in genotyping.  This ambiguity is caused by the inability of most assays to distinguish which copy of the genome contributed the polymorphism (mother's or father's). The combination of polymorphic differences between the two distinct copies of the genome (haplotypes), can not easily be resolved by ordinary DNA analysis.

Step 1: Specially designed probes and reagents are added to a diploid genomic DNA sample.

Step 2: The probes target a unique DNA sequence, such as a heterozygous SNP, through denaturing of the DNA and probe elongation.

Step 3: The targeted DNA is then captured by magnetic beads on an automated system.

Step 4: The captured DNA, which can be either haploid or diploid depending upon the probe design or combination of probes, is purified during a washing step.

Step 5: The purified DNA is then analyzed by conventional methods.

For some assays, such as quantitative PCR, the magnetic beads should be removed from the DNA by a simple heating step and bead removal (Step 5a).

Works with all types of down stream assays

Our technology has successfully been proven to work with the following  biochemical assays:

 Array CGH (comparative genomic hybridization)
 DNA sequencing
 Next-Generation Sequencing
 Pyrosequencing
 SNP-genotyping
 Real-Time (Quantitative) PCR
 SSO DNA arrays
 SSP allele-specific PCR

Developed for common automated systems

HSE has been demonstrated on the following platforms:

 EZ-1/Geno M-6 robotic system (Qiagen)
 Tecan Genesis
 Kingfisher/BioSprint 96 (Thermo Electron/Qiagen)