Technical Literature and User Guides
Technical Presentation
EFI 2007
Enrichment of Specific Chromosomal Targets by Region Specific Extraction (RSE)
Technical Posters
HGV 2008
Parallel, Cost-effective and Easily Reconfigurable Isolation of Genomic Regions
EFI 2007
Haplotype Specific Isolations from Genomic DNA using a High Throughput System
HGV 2006
Tracking of Movable Genomic Elements and Copy Number Variations by Sequence Specific Capture and Microarray Analysis
HGV 2006
Evaluation of Haplotype Specific Enrichment (HSE) for Phase Resolution of Multiple SNPs in the CFTR Locus
YMGB 2006
Changes in Retro-transposon Organization in the Saccharomyces Cerevisiae Genome During Natural and Experimental Evolution
ASHG 2005
Multiplexed Haplotype Specific Extraction (HSE) of Genomic DNA
Oakridge 2005
Haploseparation and Amplification of Genomic DNA by Haplotype Specific Extraction (HSE) and Whole Genome Amplification
User Manuals
HaploPrep(Qiagen)
HLA-A Probe Selection Handbook
HLA-B Probe Selection Handbook
HLA-C Probe Selection Handbook
HLA-DR Probe Selection Handbook
HLA-DQB1 Probe Selection Handbook
HaploPrep User Manual for EZ1
Selected Peer-Reviewed Publications
SNP-specific extraction of haplotype-resolved targeted genomic regions.
Dapprich J, Ferriola D, Magira EE, Kunkel M, Monos D. Nucleic Acids Res. 2008 Jul 8. PMID: 18611953
Identification of a Novel HLA-DPB1 Allele (DPB1*1902) by Haplotype Specific Extraction and Nucleotide Sequencing.
J. Dapprich, Eleni Magira, Mary Ann Samonte, Curt Lind, Milton D. Rossman, Kenneth Rosenman, Susan Hsu, Dimitri Monos. (2007) Tissue Antigens, 69:282-283
Haplotype Specific Extraction: A Universal Method to Resolve Ambiguous Genotypes and Detect New Alleles –Demonstrated on HLA-B.
M. Nagy, P. Entz, P. Otremba, C. Schoenemann, N. Murphy, J. Dapprich, (2007) Tissue Antigens 69:176-180
Global Mapping of Transposon Location.
A. Gabriel, J. Dapprich, M. Kunkel, D. Gresham, S. Pratt, M. Dunham, (2006) PLoS Genetics, Vol. 2, No. 12, e212 p.1-13
HLA-B*1586 is a novel, hybrid HLA-B15/B22 allele with unique serology and haplotypic association. Yang, Y., Aubrey, M. T., Zhang, G., Ji, Y. & Freed, B. M. (2006) Tissue Antigens 67 (2), 176-177. doi: 10.1111/j.1399-0039.2006.00531.x
The recombinant HLA-B*5518 allele supports the evidence of conserved haplotype association of rare alleles. Lebedeva, T.V., Huang, A., Ohashi, M., Sibilia, P., Alosco, S.M., Kempenich, J. & Yu, N. (2005) Tissue Antigens 66 (2), 156-159. doi: 10.1111/j.1399-0039.2005.00442.x
Ambiguous allele combinations in HLA Class I and Class II sequence-based typing: when precise nucleotide sequencing leads to imprecise allele identification. Sharon D Adams, Kathleen C Barracchini, Deborah Chen, FuMeei Robbins, Lu Wang, Paula Larsen, Robert Luhm, and David F Stroncek J Transl Med. 2004; 2: 30. Published online 2004 September 13. doi: 10.1186/1479-5876-2-30.
A novel HLA-B*420502 allele identified by PCR-SSO/SSP routine typing and confirmed by Sequencing-based typing*. Mrazek, F., Fae, I., Ambruzova, Z., Raida, L., Indrak, K., Petrek, M. & Fischer, G.F. (2005)
Tissue Antigens 65 (3), 275-277. doi: 10.1111/j.1399-0039.2005.00376.x
Identification of an HLA-B*07 allele variant (B*0740) in the Chinese Han population. Wang, C.Y., Pan, Q., Xue, M., Miao, K.R., Fei, X.M., Zhou, X.Y., Zhao, X., KuKuruga, D., Osowski, L., Poore, B., Beattie, R., Shi, W.X. & Zhang, H. (2005) Tissue Antigens 66 (2), 148-150. doi: 10.1111/j.1399-0039.2005.00438.x
B*4440: a novel HLA-B allele identified by sequence-specific oligonucleotide hybridization and sequence-specific amplification.
G.A. Heymann, B. Hoppe, M. Nagy, C. Schoenemann, H. Kiesewetter, A. Salama Vol. 65 Issue 2 Page 195 February 2005
A Rapid, Automatable Method For Molecular Haplotyping.
J. Dapprich, M.A. Cleary, H.W. Gabel, A. Akkapeddi, B. Iglehart, C. Turino, L. Beaudet, J. Lian, N.B. Murphy. HLA 2004: Immunobiology of the Human MHC. Proceedings of the 13th International Histocompatibility Workshop and Congress. (Hansen JA and Dupont B, eds), Volume I & II, IHWG Press, Seattle, WA, 2004. ISBN: 0-945278-03-9
